Gamma ray-giving off radionuclides from the samples encapsulated in the U8 pots was indeed measured having fun with a germanium semiconductor detector (Canberra GC2020, GC3020 and GC4020; Mirion Innovation (Canberra) KK, Tokyo, Japan) linked to a multichannel analyser.
Research regarding physicochemical portions
The newest physicochemical portions away from 137 Cs throughout the belly information was investigated by the group with the pursuing the about three portions in accordance with the physicochemical portions out-of 137 Cs established in the floor: (1) a keen exchangeable tiny fraction; (2) a pledged so you can organic amount fraction; and you can (3) a strongly likely small fraction. We presumed one (1) the brand new exchangeable fraction is restricted seemingly broadly which will be immersed within the the fresh digestive system; (2) new bound to normal matter fraction will normal matter which will be partly absorbed during digestive; and you will (3) the newest strongly sure fraction is excreted without being absorbed toward system.
One to gram away from dehydrated test was put into ten mL from step 1 M ammonium nitrate services hoe werkt eris (which have a good–drinking water ratio of just one:10) and you will influenced for 1 time, followed closely by filter using good 0.twenty two ?m membrane layer filter (MilliporeSigma, Burlington, MA, USA) The fresh filtrate who has only the 137 Cs exchangeable fraction ended up being recovered and you will encapsulated from inside the a great U8 container.
Just after modifying this new good–liquid ratio to a single:seven.5, dos.5 mL from step three.dos M ammonium acetate solution (20% nitric acidic) try set in pull the fresh 137 Cs (with a good–water ratio of 1:10). The new deposits had been up coming additional and you will rinsed in identical matter (ten mL) away from filtered liquids, followed by mix for the removal water. Because filtrate consisted of the fresh new exchangeable and you can destined to natural count portions, the activity intensity of the new 137 Cs regarding bound to organic count small fraction is actually determined of the deducting the new concentration of 137 Cs on exchangeable tiny fraction (received when you look at the (1)) out of regarding 137 Cs in the filtrate.
The game intensity of the newest 137 Cs regarding the firmly-sure fraction are calculated by the addition of the game concentration of 137 Cs regarding exchangeable small fraction inside (1) and this on the bound to all-natural matter small fraction from inside the (2) significantly more than, then subtracting that it full from the passion intensity of 137 Cs obtained to the dried belly blogs products.
Analytical research
I performed all the analytical analyses playing with JMP thirteen.dos.step one application (SAS, Cary, NC, USA). The newest relationship between the craft density from 137 Cs inside the muscles along with per physicochemical fraction of tummy material (Fig. 2), new big date regarding grab therefore the interest concentration of 137 Cs into the strength otherwise belly articles (Fig. 3) and go out regarding grab as well as the craft concentrations off 137 Cs of each physicochemical tiny fraction (Fig. 3) was basically in addition to analysed. Additionally, just like the region or all the destined to all-natural number tiny fraction is absorbed during digestion, i including analysed the sum total exchangeable and you may destined to organic matter portions (Figs. dos and you can 4)
Matchmaking between the pastime intensity of radiocaesium ( 137 Cs) in wild boar muscle mass and also in additional fractions regarding belly articles. This new unmarried regression range picture, modified Roentgen-square (R 2 ) and you can correlation coefficient (r) are given simply for brand new cases where a change is seen among them variables because of the regression and you will correlation analyses. DW: Deceased weight, FM: New size.
Regular variations in the game intensity of radiocaesium ( 137 Cs) inside the wild boar muscle mass and you can belly contents. (good,b) Monthly alterations in the activity concentration of 137 Cs in the muscle and you can stomach content material out-of . Top of the and lower closes of box patch mean the fresh 75th and you may 25th percentiles, correspondingly. The brand new lateral pub from the plot indicates the average worth. The brand new whiskers within upper minimizing finishes of your box spot indicate the maximum and you can lowest philosophy, respectively. (c,d) Changes in the activity intensity of 137 Cs inside muscle and you can belly content material into times if animals was caught. Brand new solitary regression range equation and you can modified R-rectangular (R 2 ) are provided only for the new instances when an improvement is seen between them variables for the regression analysis. FM: Fresh bulk.