To your chromosome 4, all the about three QTLs was in fact during the 66
Container plots of land exhibiting the effects of your own marker genotypic groups (AAAA, AAAB, AABB, ABBB, BBBB) off 4 candidate SNPs towards the tuber glucose amount (GLU); bud-end fry colour (FBE); stem-tuber fry colour (FSE); ID, Idaho
This new co-localization from numerous QTLs on maturity locus part in this studies is consistent with the noticed designs from phenotypic correlations. 01) with quite a few attributes overlapping an equivalent hereditary reputation (Fig. 1). Including, EBID11 and you can VWID11 score both showed a powerful negative correlation that have MAT95ID and MAT120ID. It development will get give an explanation for biggest effect QTL with afterwards maturity closely from the put off onset of VW and you can EB, or simply, very early maturity ultimately causing early onset of VW and you can EB. A relatively higher correlation has also been observed ranging from vine readiness and you can increases behavior, having early maturity regarding the more spread development behavior. Having said that datingranking.net/escort-directory/costa-mesa, a reduced correlation (roentgen ? 0.2) anywhere between vine readiness and you may both tuber sugar amount and you can bud-avoid fry color are consistent with the latest models of for the QTL effects.
Tuber glucose, bud-end and you can stalk-avoid fry colour
Along with chromosome 5, extreme QTLs having glucose, bud-end and you may base-prevent fry colour was basically observed within the overlapping aspects of chromosomes cuatro, 6, ten, and you can eleven. 0 cM on LOD scores anywhere between cuatro.5 to 6.0 (Table step 3, Fig. 4). These QTLs explained ranging from seven.dos and you will eleven.3% of one’s trait variance. Study of different simple models indicated just one better-fitted model for everyone three faculties, with a dual-simplex allele (AAAB ? AAAB) towards homologous chromosomes H2 and you can H5 out-of RGR and you may Advertising, respectively (Table step 3, Extra document 8: Contour S4). To have glucose, the B allele is of this higher glucose concentration, if you’re to own bud-end and you may stalk-end fry colour, the current presence of the latest B allele conveyed all the way down reflectance values (Fig. 3b).
LOD rating shipment away from QTLs for three faculties across the twelve potato chromosomes (Chr). Abbreviations are as with Fig. step one
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
seven Mb) that features numerous genetics of this starch/sugar inter-transformation plus hexokinase (HXK-six, PGSC0003DMG400016521), fructokinase (FRK-6/1, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and invertase (INV-na-6/1, PGSC0003DMG400026107, INV-6/2, PGSC0003DMG400033142) genes.